Tuesday, 24 October 2017

Result

Leaf extraction from Ficus deltoidea

About one kilogram fresh plant materials was used in this experiment and about 50 grams of leaf powder were obtained. Then after extraction, about five grams of dried extract were obtained with a modification of the procedures where the extraction was done by methanol.

The effect of Ficus deltoidea extract on Ganoderma boninense 

From the result, plates containing F. deltoidea extract showed antifungal activities against G. boninense. The mycelial growth of G. boninense was inhibited 55.9% at 5% concentration and 100% inhibited at 10,15 and 20% concentrations.

Percentage inhibition of diameter growth (PIDG) of F.deltoidea extract against G. boninense at 7 days after incubation. Data represents results of 3 replicates. Values are the means of 3 replicates. Means with the same letter are not significantly different at P = 0.05. 

The effect of Ficus deltoidea extract on Rhizoctonia solani 

From the result plates containing F.deltoidea extract showed antifungal activities against R.solani. The mycelial growth of R.solani was inhibited 53.67% at 5% concentration and 100% inhibited at 10,15 and 20° 0 concentrations.

Percentage inhibition of diameter growth (PIDG) of F.deltoidea extract against R.solani at 5 days after incubation. Data represents results of 3 replicates. Values are the means of 3 replicates. Means with the same letter are not significantly different at P = 0.05. 

The effect of Ficus deltoidea extract based on concentration

 From the result, plates containing F.deltoidea extract showed slightly higher inhibition on G. boninense compared to R.solani. However, for 10, 15 and 20% concentrations, the treatments inhibited 100% on both pathogens. The percentage of inhibition of G. boninense was 55,9% with R solani 53.67% at 5% concentration.

Therefore, this experiment showed that 10, 15 and 20% concentrations of F.deltoidea leaf extract could effectively stop the mycelial growth of both pathogens. Thus, 10% concentration of F. deltoidea leaf extract is recommended to be used for inhibition 
both fungal pathogens.

Comparison of percentage inhibition of diameter growth (PIDG) of Ficus deltoidea extract against Ganoderma boninense and Rhizoctonia solani based on concentration G, Ganoderma boninense; R, Rhizoctonia solani .Values are the means of 3 replicates. Means witfh the same letter are not significantly ditferent at P = 0.05


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